中文E.coli O157: H7 PCR ohun elo
Orukọ ọja
E.coli O157:H7 PCR ohun elo wiwa (Lyophilized)
Iwọn
48 igbeyewo / kit, 50 igbeyewo / kit
Lilo ti a pinnu
Escherichia coli O157:H7 (E.coli O157:H7) jẹ kokoro arun giramu-odi ti o jẹ ti iwin Enterobacteriaceae, eyiti o nmu iye nla ti majele Vero jade.Ni ile-iwosan, o maa nwaye lojiji pẹlu irora ikun ti o lagbara ati gbuuru omi, ti o tẹle pẹlu gbuuru iṣọn-ẹjẹ ni awọn ọjọ diẹ lẹhinna, eyiti o le ja si iba tabi ko si ibà, ati iku ni awọn iṣẹlẹ ti o lewu.Ohun elo yii dara fun wiwa didara ti Escherichia coli O157: H7 ninu ounjẹ, awọn ayẹwo omi, awọn ọgbẹ, eebi, omi ti o mu kokoro-arun ati awọn apẹẹrẹ miiran nipa lilo ilana ti PCR akoko gidi.The kit is an ALL-READY PCR SYSTEM( Lyophilized), eyiti o ni henensiamu ampilifaya DNA, ifipamọ ifapa, awọn alakoko kan pato ati awọn iwadii ti o nilo fun wiwa PCR fluorescent.
Ọja Awọn akoonu
| Awọn eroja | Package | sipesifikesonu | Eroja |
| E.coli O157: H7 PCR Mix | 1 × igo (lulú ti a fi Lyophilized) | 50 Idanwo | dNTPs, MgCl2, Alakoko, Awọn iwadii, Yiyipada Transcriptase, Taq DNA polymerase |
| 6× 0.2ml 8 daradara-rinhoho tube(Lyophilized) | 48 Idanwo | ||
| Iṣakoso rere | 1 * 0.2ml tube (lyophilized) | 10 Idanwo | Plasmid ti o ni E.coli O157: H7 awọn ajẹkù pato |
| Itupalẹ ojutu | 1,5 milimita Cryotube | 500uL | / |
| Iṣakoso odi | 1,5 milimita Cryotube | 200uL | 0.9% NaCl |
Ibi ipamọ & Igbesi aye selifu
(1) Ohun elo naa le gbe ni iwọn otutu yara.
(2) Igbesi aye selifu jẹ awọn oṣu 18 ni -20 ℃ ati awọn oṣu 12 ni 2℃ ~ 30℃.
(3) Wo aami lori ohun elo fun ọjọ iṣelọpọ ati ọjọ ipari.
(4) Awọn lyophilized lulú version reagent yẹ ki o wa ni ipamọ ni -20 ℃ lẹhin itu ati awọn tun didi -thaw yẹ ki o wa kere ju 4 igba.
Irinse
GENECHECKER UF-150, UF-300 gidi-akoko fluorescence PCR irinse.
Aworan atọka isẹ
a) Ẹya igo:
b) Ẹya tube tube 8 daradara:
PCr Imudara
Ti ṣe iṣeduroEto
| Igbesẹ | Yiyipo | Iwọn otutu (℃) | Aago | Fluorescence ikanni |
| 1 | 1 | 95 | ||
| 2 | 40 | 95 | 5s | |
| 60 | 10s | Gba FAM fluorescence |
* Akiyesi: Awọn ifihan agbara ti ikanni fluorescence FAM yoo gba ni 60℃.
| ikanni | Itumọ ti awọn esi |
| FAM ikanni | |
| Ct≤35 | E.coli O157: H7 Rere |
| Undet | E.coli O157: H7 Odi |
| 35 | Abajade ifura, atunwo* |
* Ti abajade idanwo ti ikanni FAM ba ni iye Ct kan ≤40 ati pe o ṣe afihan ọna imudara apẹrẹ “S” aṣoju, abajade jẹ itumọ bi rere, bibẹẹkọ o jẹ odi.
