Apo Iwari PCR Staphylococcus aureus

Apejuwe kukuru:

Staphylococcus aureus jẹ ti iwin Staphylococcus ati pe o jẹ kokoro arun ti o ni giramu.O jẹ microorganism pathogenic ti o wọpọ ti ounjẹ ti o le gbejade awọn enterotoxins ati fa majele ounje.


Alaye ọja

ọja Tags

Orukọ ọja

Staphylococcus aureus Apo Iwari PCR (lyophilized)

Iwọn

48 igbeyewo / kit, 50 igbeyewo / kit

Lilo ti a pinnu

Staphylococcus aureus jẹ ti iwin Staphylococcus ati pe o jẹ kokoro arun ti o ni giramu.O jẹ microorganism pathogenic ti o wọpọ ti ounjẹ ti o le gbejade awọn enterotoxins ati fa majele ounje.Ohun elo yii nlo ilana ti PCR Fuluorisenti gidi-akoko ati pe o dara fun wiwa didara ti Staphylococcus aureus ninu ounjẹ, awọn ayẹwo omi, awọn idọti, eebi, omi imudara ati awọn apẹẹrẹ miiran.

Ọja Awọn akoonu

Awọn eroja Package sipesifikesonu Eroja
PCR Mix 1 × igo (lulú ti a fi Lyophilized)  50 Idanwo dNTPs, MgCl2, Alakoko, Awọn iwadii, Taq DNA polymerase
6× 0.2ml 8 daradara-rinhoho tube(Lyophilized) 48 Idanwo
Iṣakoso rere 1 * 0.2ml tube (lyophilized)  10 Idanwo

Plasmid tabi Pseudovirus ti o ni awọn ajẹkù kan pato ninu

Itupalẹ ojutu 1,5 milimita Cryotube 500uL /
Iṣakoso odi 1,5 milimita Cryotube 100uL 0.9% NaCl

Ibi ipamọ & Igbesi aye selifu

(1) Ohun elo naa le gbe ni iwọn otutu yara.

(2) Igbesi aye selifu jẹ awọn oṣu 18 ni -20 ℃ ati awọn oṣu 12 ni 2℃ ~ 30℃.

(3) Wo aami lori ohun elo fun ọjọ iṣelọpọ ati ọjọ ipari.

(4) Awọn lyophilized lulú version reagent yẹ ki o wa ni ipamọ ni -20 ℃ lẹhin itu ati awọn tun didi -thaw yẹ ki o wa kere ju 4 igba.

Irinse

GENECHECKER UF-150, UF-300 gidi-akoko fluorescence PCR irinse.

Aworan atọka isẹ

a) Ẹya igo:

1

b) Ẹya tube tube 8 daradara:

2

PCr Imudara

Eto ti a ṣe iṣeduro

Igbesẹ

Yiyipo

Iwọn otutu (℃)

Aago

Fluorescence ikanni

1

1

95

2 min

/

2

40

95

6s

/

60

12s

Gba FAM fluorescence

Awọn abajade Idanwo Itumọ

Channel

Itumọ ti awọn esi

FAMChannel

Ct≤35

Staphylococcus aureus Rere

Undet

Staphylococcus aureus Odi

35<Ct40

Ifuraesi,idanwo*

*Ti o ba jẹawọn esi atunwo ti ikanni FAMhasiye Ct kan ≤40ati ki o fihan aṣoju"Sapẹrẹ ampilifaya ti tẹ, abajade jẹ itumọ bi rere, bibẹẹkọ o jẹ odi.


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