中文Apo Iwari PCR Listeria monocytogenes
Orukọ ọja
Apo Iwari PCR Listeria monocytogenes
Iwọn
48 igbeyewo / kit, 50 igbeyewo / kit
Lilo ti a pinnu
Listeria monocytogenes jẹ microbacterium rere ti giramu ti o le dagba laarin 4℃ ati 45℃.O jẹ ọkan ninu awọn pathogens akọkọ ti o ṣe idẹruba ilera eniyan ni ounjẹ ti o tutu.Awọn ifihan akọkọ ti ikolu jẹ septicemia, meningitis ati mononucleosis.Ohun elo yii dara fun wiwa didara ti awọn monocytogenes Listeria ninu ounjẹ, awọn ayẹwo omi, awọn idọti, eebi, omi ti o mu kokoro-arun ati awọn apẹẹrẹ miiran nipa lilo ilana ti PCR fluorescence gidi-akoko.Kit naa jẹ eto PCR GBOGBO-SETAN (Lyophilized) , eyiti o ni henensiamu ampilifaya DNA, yiyipada transcriptase, ifasilẹ ifasilẹ, awọn alakoko kan pato ati awọn iwadii ti o nilo fun wiwa PCR fluorescent.
Ibi ipamọ & Igbesi aye selifu
(1) Ohun elo naa le gbe ni iwọn otutu yara.
(2) Igbesi aye selifu jẹ awọn oṣu 18 ni -20 ℃ ati awọn oṣu 12 ni 2℃ ~ 30℃.
(3) Wo aami lori ohun elo fun ọjọ iṣelọpọ ati ọjọ ipari.
(4) Awọn lyophilized lulú version reagent yẹ ki o wa ni ipamọ ni -20 ℃ lẹhin itu ati awọn tun didi -thaw yẹ ki o wa kere ju 4 igba.
Ọja Awọn akoonu
| Awọn eroja | Package | sipesifikesonu | Eroja |
| Listeria monocytogenes PCR Mix | 1 × igo (lulú ti a fi Lyophilized) | 50 Idanwo | dNTPs, MgCl2, Alakoko, Awọn iwadii, Yiyipada Transcriptase, Taq DNA polymerase |
| 6× 0.2ml 8 daradara-rinhoho tube(Lyophilized) | 48 Idanwo | ||
| Iṣakoso rere | 1 * 0.2ml tube (lyophilized) | 10 Idanwo | Plasmid ti o ni Listeria monocytogenes ninu awọn ajẹkù kan pato |
| Itupalẹ ojutu | 1,5 milimita Cryotube | 500uL | / |
| Iṣakoso odi | 1,5 milimita Cryotube | 200uL | 0.9% NaCl |
Irinse
GENECHECKER UF-150, UF-300 gidi-akoko fluorescence PCR irinse.
Aworan atọka isẹ
a)
b)
PCr Imudara
Ti ṣe iṣeduroEto
| Igbesẹ | Yiyipo | Iwọn otutu (℃) | Aago | Fluorescence ikanni |
| 1 | 1 | 50 | 8 min | |
| 2 | 1 | 95 | 2 min | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s | Gba FAM fluorescence |
* Akiyesi: Awọn ifihan agbara ti awọn ikanni fluorescence FAM yoo gba ni 60℃.
Awọn abajade Idanwo Itumọ
| ikanni | Itumọ ti awọn esi |
| FAM ikanni | |
| Ct≤35 | Listeria monocytogenes Rere |
| Undet | Listeria monocytogenes Negetifu |
| 35 | Abajade ifura, atunwo* |
* Ti abajade idanwo ti ikanni FAM ba ni iye Ct kan ≤40 ati pe o ṣe afihan ọna imudara apẹrẹ “S” aṣoju, abajade jẹ itumọ bi rere, bibẹẹkọ o jẹ odi.
