Ohun elo Iwari Salmonella PCR

Apejuwe kukuru:

Salmonella jẹ ti Enterobacteriaceae ati Giramu-odi enterobacteria.Salmonella jẹ pathogen ti o wọpọ ti ounjẹ ati awọn ipo akọkọ ni majele ounje kokoro arun.


Alaye ọja

ọja Tags

Orukọ ọja

Ohun elo Iwari Salmonella PCR (lyophilized)

Iwọn

48 igbeyewo / kit, 50 igbeyewo / kit

Lilo ti a pinnu

Salmonella jẹ ti Enterobacteriaceae ati Giramu-odi enterobacteria.Salmonella jẹ pathogen ti o wọpọ ti ounjẹ ati awọn ipo akọkọ ni majele ounje kokoro arun.Awọn aami aiṣan akọkọ ti majele ounjẹ ti o ṣẹlẹ nipasẹ Salmonella jẹ ríru, ìgbagbogbo, irora inu, orififo, otutu ati gbuuru.Ohun elo yii nlo ilana ti PCR Fuluorisenti gidi-akoko ati pe o dara fun wiwa agbara ti Salmonella ninu ounjẹ, awọn ayẹwo omi, idọti, eebi, ati omi imudara.

Ọja Awọn akoonu

Awọn eroja Package sipesifikesonu Eroja
PCR Mix 1 × igo (lulú ti a fi Lyophilized)  50 Idanwo dNTPs, MgCl2, Alakoko, Awọn iwadii, Taq DNA polymerase
6× 0.2ml 8 daradara-rinhoho tube(Lyophilized) 48 Idanwo
Iṣakoso rere 1 * 0.2ml tube (lyophilized)  10 Idanwo

Plasmid tabi Pseudovirus ti o ni awọn ajẹkù kan pato ninu

Itupalẹ ojutu 1,5 milimita Cryotube 500uL /
Iṣakoso odi 1,5 milimita Cryotube 100uL 0.9% NaCl

Ibi ipamọ & Igbesi aye selifu

(1) Ohun elo naa le gbe ni iwọn otutu yara.

(2) Igbesi aye selifu jẹ awọn oṣu 18 ni -20 ℃ ati awọn oṣu 12 ni 2℃ ~ 30℃.

(3) Wo aami lori ohun elo fun ọjọ iṣelọpọ ati ọjọ ipari.

(4) Awọn lyophilized lulú version reagent yẹ ki o wa ni ipamọ ni -20 ℃ lẹhin itu ati awọn tun didi -thaw yẹ ki o wa kere ju 4 igba.

Irinse

GENECHECKER UF-150, UF-300 gidi-akoko fluorescence PCR irinse.

Aworan atọka isẹ

a) Ẹya igo:

1

b) Ẹya tube tube 8 daradara:

2

PCr Imudara

Eto ti a ṣe iṣeduro

Igbesẹ

Yiyipo

Iwọn otutu (℃)

Aago

Fluorescence ikanni

1

1

95

2 min

/

2

40

95

5s

/

60

10s

Gba FAM fluorescence

Awọn abajade Idanwo Itumọ

ikanni

Itumọ ti awọn esi

FAM ikanni

Ct≤35

Salmonella Rere

Undet

Salmonella Negetifu

35

Abajade ifura, atunwo*

* Ti abajade idanwo ti ikanni FAM ba ni iye Ct kan ≤40 ati pe o ṣe afihan ọna imudara apẹrẹ “S” aṣoju, abajade jẹ itumọ bi rere, bibẹẹkọ o jẹ odi.


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