中文Apoti ibisi ati ọlọjẹ aarun atẹgun RT-PCR Apo
Orukọ ọja
Ẹran ibisi ati ọlọjẹ aarun atẹgun ti RT-PCR ohun elo wiwa (Lyophilized)
Iwọn
48 igbeyewo / kit, 50 igbeyewo / kit
Lilo ti a pinnu
Ohun elo yii nlo ọna fluorescent RT-PCR gidi-akoko lati ṣe awari RNA ti ibisi Porcine ati ohun elo wiwa aarun atẹgun nucleic acid (PRRSV) ninu awọn ohun elo aarun ara gẹgẹbi awọn tonsils, awọn apa omi-ara ati ọlọ ati awọn ohun elo aarun olomi gẹgẹbi ajesara ati ẹjẹ ti elede.O dara fun wiwa, iwadii aisan ati iwadii ajakale-arun ti Porcine Blue Ear Iwoye.Ohun elo naa jẹ eto PCR GBOGBO ti o ti ṣetan (Lyophilized), eyiti o ni iyipada transcriptase, enzymu ampilifaya DNA, ifipamọ ifasẹyin, awọn alakoko kan pato ati awọn iwadii ti a beere fun wiwa fluorescent RT-PCR.
Ọja Awọn akoonu
| Awọn eroja | Package | sipesifikesonu | Eroja |
| PBEV PCR Mix | 1 × igo (lulú ti a fi Lyophilized) | 50 Idanwo | dNTPs, MgCl2, Alakoko, Awọn iwadii, Yiyipada Transcriptase, Taq DNA polymerase |
| 6× 0.2ml 8 daradara-rinhoho tube(Lyophilized) | 48 Idanwo | ||
| Iṣakoso rere | 1 * 0.2ml tube (lyophilized) | 10 Idanwo | Plasmid tabi Pseudovirus ti o ni awọn ajẹkù pato PRRSV ninu |
| Itupalẹ ojutu | 1,5 milimita Cryotube | 500uL | / |
| Iṣakoso odi | 1,5 milimita Cryotube | 200uL | 0.9% NaCl |
Ibi ipamọ & Igbesi aye selifu
(1) Ohun elo naa le gbe ni iwọn otutu yara.
(2) Igbesi aye selifu jẹ awọn oṣu 18 ni -20 ℃ ati awọn oṣu 12 ni 2℃ ~ 30℃.
(3) Wo aami lori ohun elo fun ọjọ iṣelọpọ ati ọjọ ipari.
(4) Awọn lyophilized lulú version reagent yẹ ki o wa ni ipamọ ni -20 ℃ lẹhin itu ati awọn tun didi -thaw yẹ ki o wa kere ju 4 igba.
Irinse
GENECHECKER UF-150, UF-300 gidi-akoko fluorescence PCR irinse.
Aworan atọka isẹ
a) Ẹya igo:
b) Ẹya tube tube 8 daradara:
PCr Imudara
Eto ti a ṣe iṣeduro
| Igbesẹ | Yiyipo | Iwọn otutu (℃) | Aago | Fluorescence ikanni |
| 1 | 1 | 48 | 8 min | / |
| 2 | 1 | 95 | 2 min | / |
| 3 | 40 | 95 | 5s | / |
| 60 | 10s | Gba FAM fluorescence |
* Akiyesi: Awọn ifihan agbara ti awọn ikanni fluorescence FAM yoo gba ni 60℃.
Awọn abajade Idanwo Itumọ
| ikanni | Itumọ ti awọn esi |
| FAM ikanni | |
| Ct≤35 | Vibrio Parahaemolyticus Rere |
| Undet | Vibrio Parahaemolyticus Negetifu |
| 35 | Abajade ifura, atunwo* |
* Ti abajade idanwo ti ikanni FAM ba ni iye Ct kan ≤40 ati pe o ṣe afihan ọna imudara apẹrẹ “S” aṣoju, abajade jẹ itumọ bi rere, bibẹẹkọ o jẹ odi.
