中文Porcine ajakale gbuuru kokoro RT-PCR erin kit
Orukọ ọja
Kokoro gbuuru ajakale-arun ẹlẹdẹ RT-PCR Apo Iwari (Lyophilized)
Iwọn
48 igbeyewo / kit, 50 igbeyewo / kit
Lilo ti a pinnu
Ohun elo yii nlo ọna fluorescent RT-PCR gidi-akoko lati ṣe awari RNA ti ọlọjẹ ajakale-arun Porcine (PEDV) ninu awọn ohun elo arun ti ara gẹgẹbi awọn tonsils, awọn apa iṣan ati ọlọ ati awọn ohun elo aarun olomi gẹgẹbi ajesara ati ẹjẹ ẹlẹdẹ.O dara fun wiwa, iwadii aisan ati iwadii ajakale-arun ti kokoro gbuuru ajakale-arun Porcine.Ohun elo naa jẹ eto PCR GBOGBO ti o ti ṣetan (Lyophilized), eyiti o ni iyipada transcriptase, enzymu imudara DNA, ifipamọ ifasẹyin, awọn alakoko kan pato ati awọn iwadii ti o nilo fun wiwa Fluorisenti RT-PCR.
Ọja Awọn akoonu
| Awọn eroja | Package | sipesifikesonu | Eroja |
| PEDV PCR Mix | 1 × igo (lulú ti a fi Lyophilized) | 50 Idanwo | dNTPs, MgCl2, Alakoko, Awọn iwadii, Taq DNA polymerase |
| 6× 0.2ml 8 daradara-rinhoho tube(Lyophilized) | 48 Idanwo | ||
| Iṣakoso rere | 1 * 0.2ml tube (lyophilized) | 10 Idanwo | Plasmid tabi Pseudovirus ti o ni awọn ajẹkù pato PEDV ninu |
| Itupalẹ ojutu | 1,5 milimita Cryotube | 500uL | / |
| Iṣakoso odi | 1,5 milimita Cryotube | 200uL | 0.9% NaCl |
Ibi ipamọ & Igbesi aye selifu
(1) Ohun elo naa le gbe ni iwọn otutu yara.
(2) Igbesi aye selifu jẹ awọn oṣu 18 ni -20 ℃ ati awọn oṣu 12 ni 2℃ ~ 30℃.
(3) Wo aami lori ohun elo fun ọjọ iṣelọpọ ati ọjọ ipari.
(4) Awọn lyophilized lulú version reagent yẹ ki o wa ni ipamọ ni -20 ℃ lẹhin itu ati awọn tun didi -thaw yẹ ki o wa kere ju 4 igba.
Irinse
GENECHECKER UF-150, UF-300 gidi-akoko fluorescence PCR irinse.
Aworan atọka isẹ
a) Ẹya igo:
b) Ẹya tube tube 8 daradara:
PCr Imudara
Eto ti a ṣe iṣeduro
| Igbesẹ | Yiyipo | Iwọn otutu (℃) | Aago | Fluorescence ikanni |
| 1 | 1 | 48 | 8 min | / |
| 2 | 1 | 95 | 2 min | / |
| 3 | 40 | 95 | 5s | / |
| 60 | 10s | Gba FAM fluorescence |
* Akiyesi: Awọn ifihan agbara ti awọn ikanni fluorescence FAM yoo gba ni 60℃.
Awọn abajade Idanwo Itumọ
| ikanni | Itumọ ti awọn esi |
| FAM ikanni | |
| Ct≤35 | PEDV Rere |
| Undet | PEDV Odi |
| 35 | Abajade ifura, atunwo* |
* Ti abajade idanwo ti ikanni FAM ba ni iye Ct kan ≤40 ati pe o ṣe afihan ọna imudara apẹrẹ “S” aṣoju, abajade jẹ itumọ bi rere, bibẹẹkọ o jẹ odi.
